高效液相色谱法快速检测纸杯中甲醛残留

Rapid Determination of Formaldehyde in Paper Cups by High Performance Liquid Chromatography

中文关键词:  纸杯  甲醛残留  高效液相色谱  水蒸气蒸馏提取

Key Words:paper cups  formaldehyde  high performance liquid chromatography  steam distillation extraction

中文摘要:

      探讨建立了纸杯中甲醛残留的高效液相色谱快速检测方法。纸杯样品经水蒸气蒸馏提取，蒸馏提取液在60℃乙酸条件下与2-4-二硝基苯肼衍生30 min，然后用高效液相色谱仪测定纸杯样品中的甲醛残留，并采用外标法定量分析。结果表明，纸杯中甲醛的定量限为2.0 mg/kg；甲醛标准曲线线性范围在0.01～5.0 mg/L内线性良好，相关系数为0.9994以上；纸杯样品在3个甲醛添加水平下的回收率范围为77.2%～95.7%，相对标准偏差（RSD）为0.9%～3.1%。该方法简便、快速、高效，可用于纸杯中甲醛残留的检测分析。

Abstract:

      A method was established for rapid determination of formaldehyde in paper cups by high performance liquid chromatography（HPLC）. After extracting with steam distillation， the sample was added with 2-4-dinitrophenylhydrazine under 60℃in acidic condition for 30 min， and detected by HPLC， and an external standard method was used for quantitative analysis. The results indicated that the limit of quantitation （LOQ） for formaldehyde was 2.0 mg/kg. The range of 0.01～5.0 mg/L for formaldehyde standard had good linear relationship （r>0.9994）. The average recoveries （n=6） of formaldehyde in paper cup samples at three added levels of LOQ ranged from 77.2%～95.7% with the relative standard deviations （RSDs） between 0.9%～3.1%. The method was convenient， quick and efficient， which could satisfy the demand of the determination of formaldehyde in paper cups．