不同木质素对酶蛋白的吸附影响及酶活变化研究

Adsorption Effects of Lignin on Different Enzyme Proteins

中文关键词:  木质素  纤维素酶  聚木糖酶  β-葡萄糖苷酶  吸附作用

Key Words:lignin  cellulase  xylanase  β-glycosidase  adsorption interaction

中文摘要:

      通过3，5-二硝基水杨酸（DNS）和对硝基苯基-β-D-吡喃葡萄糖苷（pNPG）比色法，研究对比碱法和水溶预处理桉木得到的两种不同木质素对不同酶蛋白（纤维素酶、聚木糖酶和β-葡萄糖苷酶）的吸附和脱附影响差异，以及木质素吸附对不同酶蛋白活性的抑制影响。两种木质素对不同酶蛋白吸附实验表明，温度对碱木质素吸附酶蛋白能力的影响大于二甲苯磺酸钠（SXS）木质素，被吸附的酶蛋白更容易从SXS木质素上脱附下来；木质素吸附会显著降低酶活，升高温度可以提高木质素对酶蛋白的吸附能力，但会抑制酶活。

Abstract:

      Two kinds of lignin obtained from alkali and water soluble pretreatment of eucalyptus were used as raw materials， and through DNS and pNPG colorimetry， the effects of two kinds of lignin on the adsorption and desorption of different enzyme proteins （cellulase， xylanase， and β-glucosidase） and the inhibition of lignin adsorption on the activities of different enzyme proteins were studied. The results showed that the effect of temperature on the adsorption capacity of alkali lignin was greater than that of odium xylene sulfonate （SXS） lignin. The experiments of different enzyme proteins adsorbed on two kinds of lignin showed that the adsorbed enzyme proteins on SXS lignin were more easily to be desorbed. After lignin adsorption， the enzyme activity was significantly reduced， and the higher temperature could not only enhance the adsorption capacity of the lignins to enzyme proteins， but also inhibit the activity of enzyme.