低酸喷雾协同蒸汽爆破预处理对小麦秸秆结构及酶水解的影响

Effects of Low-acid Spraying Combined with Steam Explosion Pretreatment on Structure and Enzyme Hydrolysis of Wheat Straw

中文关键词:  小麦秸秆  硫酸  喷雾  蒸汽爆破  酶水解

Key Words:wheat straw  sulfuric acid  spraying  steam explosion  enzyme hydrolysis

中文摘要:

      研究了低酸喷雾协同低温蒸汽爆破（以下简称汽爆）预处理条件对小麦秸秆纤维的化学组分、结构及酶水解效率的影响。结果表明，在5%硫酸浓度（料液比1∶1）、汽爆反应温度170 ℃（0.79 MPa）、反应时间5 min预处理条件下，纤维素保留率达91.3%，半纤维素脱除率达83.4%，水解液糖得率为80.1%；对预处理后小麦秸秆进行酶水解反应72 h，可获得84.9%的葡萄糖酶解率。本研究提出的低温汽爆预处理农业秸秆的方法，可实现纤维素的高保留率并获得优异的酶水解效率。

Abstract:

      Low-acid spraying combined with low-temperature steam explosion was used to pretreat wheat straw in this study. The effects of pretreatment conditions on components， structure， and enzymatic hydrolysis efficiency of wheat straw were investigated. The results showed that cellulose retention rate reached 91.3% with hemicellulose removal rate of 83.4% and sugar yield in hydrolysate of 80.1% when wheat straw was sprayed with 5% sulfuric acid （solid to liquid ratio 1∶1） at 170 ℃ （0.79 MPa） for 5 min. In addition， the enzymatic hydrolysis rate of glucose was 84.9% after 72 h of enzymatic hydrolysis. This study proposed a low-temperature steam explosion pretreatment method for wheat straw， which achieved high cellulose retention and outstanding enzymatic hydrolysis efficiency.