可提高漆酶酶活的载锰活性炭-纤维素复合吸附剂的制备及应用

Preparation and Application of Manganese-loaded Activated Carbon-Cellulose Composite Adsorbent for Enhancing Laccase Enzyme Activity

中文关键词:  废弃载锰活性炭  纤维素基吸附剂  固定化漆酶  双酚A降解

Key Words:waste manganese-loaded activated carbon  cellulose-based adsorbent  immobilized laccase  bisphenol A degradation

中文摘要:

      利用废弃的载锰活性炭与纤维素，通过溶剂再生法制备了一种可用于固定化漆酶、提高固定化漆酶酶活的载锰活性炭-纤维素复合吸附剂，并用其固定化漆酶制备漆酶-载体材料。研究结果表明，Mn²⁺含量对漆酶-载体材料酶活有一定的影响，复合吸附剂中Mn²⁺含量为1.5 mg/g时，漆酶-载体材料酶活最高；漆酶固定化的最佳工艺条件为固定化温度45 ℃、pH值=4，初始酶浓度6.5 g/L；在最佳固定化反应条件下，漆酶-载体材料的漆酶固定化率最高达48.24%；漆酶-载体材料的热稳定性和pH稳定性均得到明显增强；利用漆酶-载体材料降解双酚A，降解率达68%，重复使用5次后的双酚A相对降解率仍可达51%。

Abstract:

      A novel manganese-loaded activated carbon-cellulose composite adsorbent that could immobilize laccase and increase the enzyme activity was prepared by solvent regeneration method， using waste manganese-loaded activated carbon and cellulose， and further prepared the laccase-carrier material after immobilizing laccase. The results showed that the content of Mn²⁺ had a certain effect on the enzyme activity of the laccase-carrier material， and the enzyme activity of the laccase-carrier material was the highest when the content of Mn²⁺ in the composite adsorbent was 1.5 mg/g. The optimum process conditions for the immobilization of laccase were the immobilization temperature of 45 ℃， the pH value of 4， and the initial enzyme concentration of 6.5 g/L. The immobilization ratio of the laccase-carrier material was the highest to 48.24% under the optimum conditions of immobilization； the thermal and pH stabilities of the laccase-carrier material were significantly enhanced； the degradation rate of bisphenol A using the laccase-carrier material reached 68%， and the relative degradation rate of bisphenol A was still up to 51% after 5 times of reuse.