3种木质纤维原料热水抽出物抗氧化性的研究

Study on Antioxidant Activity of Hot Water Extractives from Different Lignocellulose Materials

中文关键词:  木质纤维  热水抽出物  抗氧化性  酚羟基

Key Words:lignocellulose  hot water extractives  antioxidant activity  phenolic hydroxyl group

中文摘要:

      本研究对青皮竹、桉木和杨木3种木质纤维原料进行热水抽提处理（95 ℃，1 h，浆浓5%），比较了不同热水抽出物的抗氧化性差异，通过紫外可见分光光度计、凝胶渗透色谱仪、热解-气相色谱/质谱联用仪和傅里叶变换红外光谱仪等分析设备和方法，研究了不同木质纤维原料热水抽出物的抗氧化性及化学结构差异。结果表明，桉木热水抽出物的抗氧化性最强（半抑制浓度为51.80 mg/L），优于商业抗氧化剂2,6-二叔丁基-4-甲基苯酚（半抑制浓度为87.99 mg/L）；不同木质纤维原料热水抽出物的抗氧化性与其酚羟基含量和共轭结构相关；热水抽出物的主要组分为木质素、多糖等有机大分子合成反应过程的中间产物。

Abstract:

      In this study， three kinds of lignocellulose materials （bamboo， eucalyptus and poplar） were prepared by hot water extraction （95 ℃， 1 h， pulp consistency of 5%）， and the antioxidant activities of their hot water extractives were compared. The difference of chemical structures among three kinds of hot water extractives was studied by means of Ultraviolet spectrophotometry， Gel Permeation Chromatography， Pyrolysis-Gas Chromatography Mass and Fourier Transform Infrared. The results showed that the antioxidant activity of the hot water extractives of eucalyptus was the highest （semi-inhibitory concentration of 51.80 mg/L）， which was superior to that of commercial antioxidant 2，6-di-tert-butyl-4-methylphenol （semi-inhibitory concentration of 87.99 mg/L）. The antioxidant activities of the hot water extractives of different lignocellulose materials were related to the content of phenolic hydroxyl groups and conjugated structures. The main components of the extractives were the intermediate products during the synthesis of organic macromolecules such as lignin and polysaccharide.