基于MOFs复合木质海绵的蛋白印迹材料用于糖蛋白的高效和选择性分离

Protein Imprinted Materials Based on MOFs Composite Wood Sponges for Efficient and Selective Separation of Glycoproteins

中文关键词:  木质海绵  金属有机骨架化合物  糖蛋白  表面印迹技术  蛋白印迹聚合物

Key Words:wood sponge  metal-organic framework  glycoprotein  surface imprinting technique  protein imprinted polymer

中文摘要:

      糖蛋白的高效富集与识别对蛋白质组学相关的医学和生物研究具有重要意义，然而，高性能糖蛋白印迹材料的构筑仍是一个挑战。本研究以木质海绵@UIO-66复合材料（WCS@UIO-66）为载体，4-乙烯基苯基硼酸为共价功能单体，设计合成了辣根过氧化酶印迹材料（WCS@UIO-66@MIPs）；利用木质海绵与金属有机骨架化合物的多孔结构和丰富的官能团，促进了模板蛋白的固定化，同时降低了传质阻力。结果表明，WCS@UIO-66@MIPs不仅对辣根过氧化酶展现了超高的吸附量（182.0 mg/g）和优异的识别性（印迹因子2.68），且能在60 min内达到吸附平衡。此外，WCS@UIO-66@MIPs还可以在混合蛋白溶液中特异性捕获目标蛋白，证明了其在糖蛋白富集和识别应用中具有良好潜力。

Abstract:

      The efficient enrichment and identification of glycoproteins hold significant relevance to proteomics-related medical and biological research. However， constructing high-performance glycoprotein imprinted materials remains challenging. In this study， the wood sponge@ UIO-66 composite （WCS@UIO-66） was employed as a carrier， and 4-vinylphenylboronic acid was taken as a covalent functional monomer to design and synthesize horseradish peroxidase-imprinted materials （WCS@UIO-66@MIPs）. Utilizing the porous structure and abundant functional groups of the wood sponge and metal-organic framework， the immobilization of template proteins was facilitated while reducing the mass transfer resistance. Consequently， WCS@UIO-66@MIPs not only demonstrated exceptionally high adsorption capacity （182.0 mg/g） and excellent selectivity （Imprinting Factor=2.68） towards horseradish peroxidase， but also achieved adsorption equilibrium within 60 min. Moreover， it could specifically capture target proteins in mixed protein solutions， demonstrating its promising potential applications for glycoprotein enrichment and identification.