侯丽萍,应光国,舒 琥,赵建亮.利用EROD和GST酶评估竹子制浆造纸废水的生物毒性[J].中国造纸学报,2013,28(1):25-29 本文二维码信息
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利用EROD和GST酶评估竹子制浆造纸废水的生物毒性
Assessing Biological Toxicity of Bamboo Pulp and Paper Effluent by Determining EROD and GST Activity in Mosquitofish
  
DOI:10.11981/j.issn.1000-6842.2013.01.25
中文关键词:  造纸废水  食蚊鱼  生物毒性
Key Words:bamboo pulp and paper effluent  mosquitofish  biological toxicity tests
基金项目:广东省教育厅育苗工程项目(2012LYM_0100);广州大学新苗计划资助项目;国家自然科学基金(NSF20977092,40688001,40821003);广州大学产学研基金、创新团队基金项目。
作者单位
侯丽萍1 1.广州大学生命科学学院广东广州510006 
应光国2 2.中国科学院广州地球化学研究所有机地球化学国家重点实验室广东广州510640 
舒 琥3,* 3.广州大学华南生物多样性研究所广东广州510006 
赵建亮2 2.中国科学院广州地球化学研究所有机地球化学国家重点实验室广东广州510640 
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中文摘要:
      测定了生活于受竹子制浆造纸废水(采用“小苏打浸泡法”制浆)污染的河道中食蚊鱼活体肝脏7-乙氧基-3-异吩呛哇酮-脱乙基酶(7-ethoxyresorufino-deethylase,EROD)和谷胱甘肽硫转移酶(Glutathione-S-transferase,GST)的活性,并论证了食蚊鱼作为评价竹子制浆造纸废水生物毒性的指示生物的可行性。结果表明,食蚊鱼是比较理想的评价竹子制浆造纸废水生物毒性的指示生物,其活体EROD能有效、真实地评价竹子制浆造纸废水的安全性,GST在指示竹子制浆造纸废水生物毒性时的效果不如EROD。
Abstract:
      The biological toxicity of bamboo pulp and paper effluent was assessed by determining EROD and GST activity in mosquitofish (Gambusia affinis) exposed to the effluent. The result showed that both female and male mosquitofish exposed to the effluent had significantly higher levels of EROD activity than that of mosquitofish living in the site which had no known point sources of pollution . Mosquitofish was a suitable aquatic animal to evaluate bamboo pulp and paper effluent pollution effects. EROD activity was a more sensitive indicator than the GST activity to monitoring of bamboo pulp and paper effluent contamination.
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