UPLC-PDA法检测桉木预水解液中5-羟甲基糠醛

Determination of 5-Hydroxymethylfurfural in Pre-hydrolysate of Eucalyptus by UPLC-PDA Method

中文关键词:  5-羟甲基糠醛  超高效液相色谱  二极管阵列  预水解  桉木

Key Words:5-hydroxymethylfurfural  UPLC  photo-diode array  pre-hydrolysis  Eucalyptus

中文摘要:

      采用超高效液相色谱-二极管阵列检测器（UPLC-PDA）法检测桉木预水解液中5-羟甲基糠醛（5-HMF）。该方法检测过程中，样品无需复杂的预处理，即可在流动相为乙腈/水（20∶80，V/V）、淋洗速度为0.2 mL/min的条件下对桉木预水解液中各组分进行先洗脱分离，然后采用PDA检测器于波长283.55 nm处定量检测桉木预水解液中的5-HMF；其检出限为3.883 μg/L，相关系数为0.9998，重现检测相对标准偏差为0.2%，加标回收率为105.2%；该法具有样品预处理简单、检测快速和准确的特点，为更好地控制预水解条件、提高预水解液利用价值提供了一定的理论支撑。

Abstract:

      A method was developed to determine 5-hydroxymethylfurfural （5-HMF） in the pre-hydrolysate of Eucalyptus by Ultra Performance Liquid Chromatography-Photo-diode Array Detector （UPLC-PDA）. Without complicated pretreatment， different components in the samples were eluted and then separated with the mobile phase of acetonitrile/water （20/80，V/V） at flow rate of 0.2 mL/min， and then quantitatively detected with PDA detector at 283.55 nm. Detection limit， the correlation coefficient， relative standard deviation （RSD） and 5-HMF standard recovery rate of the determination method were 3.883 μg/L， 0.9998， 0.2% and 105.2%， respectively. The method developed had characteristics of simple sample preparation， fast determination and high accuracy. Finally， this method was used for fast determination of 5-HMF in the Eucalyptus pre-hydrolysate， which was beneficial to optimization of pre-hydrolysis conditions and utilization of pre-hydrolysate.